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For Caspase-3 staining, the tissues were blocked with 10% normal horse serum for 30 min then incubated with Caspase-3 antibody (1:300, BD Biosciences, Macquarie Park, NSW, Australia) overnight, followed by secondary antibodies (Goat anti-rabbit HRP, 1:200, Vector Laboratories, Burlingame, CA, USA) for 45 min

Lets be honest, this is the crucial part of any preliminary investigation

Because these species differ in stability, bioavailability, and pharmacological effects, the true dose of the active compound becomes difficult to determine

Levels of TNF- and soluble TNF receptors in normal-weight, overweight and obese patients with dilated non-ischemic cardiomyopathy: does Anti-TNF therapy still have potential to be used in heart failure depending on BMI